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Supervised by Ministry of Industry and Information Technology of The People's Republic of China Sponsored by Harbin Institute of Technology Editor-in-chief Yu Zhou ISSNISSN 1005-9113 CNCN 23-1378/T

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Related citation:ZHANG Jun-zheng,Yang Qian,Yang Lie.Expression of green fluoscrescent protein gene in Sclerotinia sclerotiorum[J].Journal of Harbin Institute Of Technology(New Series),2009,16(3):346-349.DOI:10.11916/j.issn.1005-9113.2009.03.009.
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Expression of green fluoscrescent protein gene in Sclerotinia sclerotiorum
Author NameAffiliation
ZHANG Jun-zheng Dept. of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China 
Yang Qian Dept. of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China 
Yang Lie Dept. of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China 
Abstract:
Protoplasts of the pathogenic plant fungus,Sclerotinia sclerotiorum,were transformed using the pPGF plasmid,which contains green fluorescent protein gene,under the control of Aspergillus nidulans regulatory sequences. The pPGF plasmid was introduced by PEG/CaCl2 treatment. Positive transformants were harvested with hygromycin B (HYG) resistance as selective marker,and then were observed with green fluorescence phenomena in response to blue light,which suggested that GFP gene was cloned into genome DNA of S. sclerotiorum. The transformants were verified mitotically stable by Southern blotting analysis and passage culturing. This study is developed as an initial step for further research into infection mechanisms of S. sclerotiorum to plants and interactions with bio-control fungus.
Key words:  sclerotinia sclerotiorum  green fluorescent protein  transformation  hygromycin resistance gene  protoplast
DOI：10.11916/j.issn.1005-9113.2009.03.009
Clc Number:S435.654
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