Please submit manuscripts in either of the following two submission systems

    ScholarOne Manuscripts

  • ScholarOne

    • 勤云稿件系统

  • 登录

Search by Issue

  • 2024 Vol.31
  • 2023 Vol.30
  • 2022 Vol.29
  • 2021 Vol.28
  • 2020 Vol.27
  • 2019 Vol.26
  • 2018 Vol.25
  • 2017 Vol.24
  • 2016 vol.23
  • 2015 vol.22
  • 2014 vol.21
  • 2013 vol.20
  • 2012 vol.19
  • 2011 vol.18
  • 2010 vol.17
  • 2009 vol.16
  • No.1
  • No.2

Search by Keywords

  • Search by Title
  • Search by Keywords
  • Search by Abstract
  • Search by Author's Chinese Name
  • Search by Author's English Name
  • Search by Fund

News & AnnouncementMORE

Supervised by Ministry of Industry and Information Technology of The People's Republic of China Sponsored by Harbin Institute of Technology Editor-in-chief Yu Zhou ISSNISSN 1005-9113 CNCN 23-1378/T

期刊网站二维码
微信公众号二维码
Related citation:ZHANG Jun-zheng,Yang Qian,Yang Lie.Expression of green fluoscrescent protein gene in Sclerotinia sclerotiorum[J].Journal of Harbin Institute Of Technology(New Series),2009,16(3):346-349.DOI:10.11916/j.issn.1005-9113.2009.03.009.
【Print】   【HTML】   【PDF download】   【View/Add Comment】  【Download reader】  【 Close 】
←Previous|Next→ Back Issue    Advanced Search
This paper has been: browsed 727times   downloaded 586times 本文二维码信息
码上扫一扫！
Shared by: Wechat More
Font:larger+|default|smaller-
Expression of green fluoscrescent protein gene in Sclerotinia sclerotiorum
Author NameAffiliation
ZHANG Jun-zheng Dept. of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China 
Yang Qian Dept. of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China 
Yang Lie Dept. of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China 
Abstract:
Protoplasts of the pathogenic plant fungus,Sclerotinia sclerotiorum,were transformed using the pPGF plasmid,which contains green fluorescent protein gene,under the control of Aspergillus nidulans regulatory sequences. The pPGF plasmid was introduced by PEG/CaCl2 treatment. Positive transformants were harvested with hygromycin B (HYG) resistance as selective marker,and then were observed with green fluorescence phenomena in response to blue light,which suggested that GFP gene was cloned into genome DNA of S. sclerotiorum. The transformants were verified mitotically stable by Southern blotting analysis and passage culturing. This study is developed as an initial step for further research into infection mechanisms of S. sclerotiorum to plants and interactions with bio-control fungus.
Key words:  sclerotinia sclerotiorum  green fluorescent protein  transformation  hygromycin resistance gene  protoplast
DOI：10.11916/j.issn.1005-9113.2009.03.009
Clc Number:S435.654
Fund:

LINKS

Copyright © The Editorial Department of Journal of Harbin Institute of Technology
Address: 92 West Dazhi Street, Nan Gang District, Harbin, Heilongjiang Province, China Postcode: 150001
(C)2015 JOURNAL OF HIT ALL RIGHTS RESERVED. ICP. 0000423255