| Related citation: | MA Fang,LI Jie-xun,WEI Li,CHEN Zhong-xi,SHAIK FIRDOZ,ZHAO Guang.Isolation and identification of a bacteria strain Enterobacter cloacae I7 for degradation of hydrolyzed polyacrylamide[J].Journal of Harbin Institute Of Technology(New Series),2009,16(5):669-672.DOI:10.11916/j.issn.1005-9113.2009.05.015. |
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| Author Name | Affiliation | | MA Fang | School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China | | LI Jie-xun | College of Petroleum Engineering,Daqing Petroleum Institute,Daqing 163318,China
Daqing Oilfield Corporation,PetroChina, Daqing 163453,China | | WEI Li | School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China | | CHEN Zhong-xi | Daqing Oilfield Corporation,PetroChina, Daqing 163453,China
Water Treatment and Oilfield Chemistry Research Department,Daqing Oilfield Engineering Co.Ltd, PetroChina,Daqing 163712,China | | SHAIK FIRDOZ | Indian Institute of Technology,Roorkee,Uttarakhand 247667,INDIA | | ZHAO Guang | School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China |
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| Abstract: |
| A bacteria strain for the degradation of hydrolyzed polyacrylamide (HPAM) was isolated from a curing pot in HPAM distribution station of Daqing Oilfield using Hungate anaerobic technique.The isolate was investigated from morphological,physiological,biochemical and molecular characterization.It is a Gram-negative,short-bacillus,non-spore-forming anaerobic bacteria with an optimum growth at 8.0 pH at 40℃.It can reduce sulfate to H2S.Alignment of 16S ribosomal DNA and 16S-23S ribosomal DNA intergenic spacer sequences suggests that this isolate is closely related to the Enterobacter cloacae.The isolate is identified as a new strain belonging to Enterobacter genus,temporarily named as Enterobacter cloacae I7.Analysis results of infrared spectroscopy (IR) show that the bacteria can use HPAM as the only carbon source,change the structure of HPAM polymer surface,and realize the hydrolysis of amide to carboxyl group by hydrolysis mechanism.It can degrade the side chain and change some functional groups,which obviously decreases the viscosity.GC-MS analysis indicates that the determined low-molecular weight degradation products of HPAM are polyacrylamide fragments with duplet bond,epoxy as well as carbonyl group,but most of them are acrylamide oligomer derivatives. |
| Key words: PAM-degrading bacteria strain Hungate anaerobic technique biodegradation Enterobacter cloacae I7 |
| DOI：10.11916/j.issn.1005-9113.2009.05.015 |
| Clc Number:X172 |
| Fund: |
