| Related citation: | XU Wei,ANG Peng,ZHANG Xing,ZHANG Yue,LIN Hai-long,SHI Xue-hua.Microwave induced mutagenesis of Lactobacillus casei subsp. rhamnosus for enhancing L-lactic acid production[J].Journal of Harbin Institute Of Technology(New Series),2009,16(5):673-677.DOI:10.11916/j.issn.1005-9113.2009.05.016. |
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| Author Name | Affiliation | | XU Wei | State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China
School of Foodstuff Engineering,Harbin University of Commerce,Harbin 150076,China | | ANG Peng | State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China | | ZHANG Xing | State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China | | ZHANG Yue | State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China | | LIN Hai-long | State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China | | SHI Xue-hua | State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China |
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| Abstract: |
| Mutagenesis of Lactobacillus casei subsp.rhamnosus X1-12 after low power microwave irradiation was investigated.Under a microwave power of 400 W and irradiation length of 3 min,a mutated strain W4-3-9 with high-yield L-lactic acid was obtained by screening.Compared with the starting strain X1-12,the L-lactic acid production of W4-3-9 was increased by 58.0% at a concentration of 115.8 g/L.The strain maintained the capability of producing a high L-lactic acid level after 10 generations.Cell surface morphology and DNA structures of parental and mutated strains were observed by atomic force microscopy (AFM).Amplified fragment length polymorphism (AFLP) analysis suggested the difference in AFLP band pattern between the mutated and non-mutated strains.Sequencing and BLAST analysis revealed that the catalytic site of lactate dehydrogenase (DHL) was changed due to the microwave induced mutation. |
| Key words: microwave irradiation Lactobacillus L-lactic acid AFM AFLP |
| DOI：10.11916/j.issn.1005-9113.2009.05.016 |
| Clc Number:X172 |
| Fund: |
