| Related citation: | WEI Li,MA Fang,WEI Ji-cheng,LI Yan-ping,LV Xiao-lei.Isolation and identification of a sulfate reducing bacteria and sequence analysis of its dissimilatory sulfite reductase gene[J].Journal of Harbin Institute Of Technology(New Series),2009,16(6):854-858.DOI:10.11916/j.issn.1005-9113.2009.06.021. |
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| Author Name | Affiliation | | WEI Li | School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090,China | | MA Fang | School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090,China | | WEI Ji-cheng | School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090,China
Mudanjiang Teachers College, Mudanjiang 157012,China | | LI Yan-ping | Mudanjiang Teachers College, Mudanjiang 157012,China | | SHAIK FIRDOZ | Indian Institute of Technoogy, Roorkee Uttarakhand, INDIA 247667 | | LV Xiao-lei | School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090,China |
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| Abstract: |
| A sulfate reducing bacteria was isolated from mining sewage of Daqing Oilfield by Hungate anaerobic technology. Physiological-biochemical analysis showed that the strain could utilize polyacrylamide as sole carbon and nitrogen source. The sequence analysis of 16S rDNA illustrated that the similarity of F8 and Desulfovibrio desulfuricans (AF192153) was 99%, and the similarity sequence of dissimilatory sulfite reductase gene (DSR) cloned from the strain and Desulfovibrio desulfuricans (AF273034) was 98%. Their phylogenitic analysis was basically anastomosed, and thus temporarily named as Desulfovibrio desulfuricans F8. The DSR cloned from F8 strain was 2740 bp in length consisting of three ORF, DSRA, DSRB and DSRD as a single operon (DSRABD) regulated by the same operator. DSRA contained typical conservative box of sulfate—sulfite reducing enzyme (SiteⅠand SiteⅡ), which could bind siroheme and . DSRB retained a binding site, with an uncomplimentary structure for siroheme binding. There was no conservative box in DSRD. Sequence analysis of DSR will provide a theoretical basis for quantitative detection, metabolic pathway modification through gene engineering, and sulfate reducing bacteria (SRB) suppression. |
| Key words: sulfate reducing bacteria DSR 16S rDNA sequence DSRABD gene sequence analysis |
| DOI：10.11916/j.issn.1005-9113.2009.06.021 |
| Clc Number:X703.1 |
| Fund: |
