| Related citation: | Junfeng Su,Sicheng Shao,Tinglin Huang,Fang Ma,Gang Wen,Shengchen Zheng,Kai Zhang.Effect of C/N Ratio,Temperature,pH on Autotrophic Denitrification Rate with Hydrogen Gas,Iron (II) and Sodium Sulfide as Electron Donors[J].Journal of Harbin Institute Of Technology(New Series),2016,23(1):90-96.DOI:10.11916/j.issn.1005-9113.2016.01.013. |
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| Effect of C/N Ratio,Temperature,pH on Autotrophic Denitrification Rate with Hydrogen Gas,Iron (II) and Sodium Sulfide as Electron Donors |
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| Author Name | Affiliation | | Junfeng Su | School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China
State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China | | Sicheng Shao | School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China | | Tinglin Huang | School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China | | Fang Ma | State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China | | Gang Wen | School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China | | Shengchen Zheng | School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China | | Kai Zhang | School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China |
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| Abstract: |
| Nitrate is considered to be one of the most widely present pollutants leading to eutrophication of environment. The purpose of this work was to isolate and identify new anaerobic denitrifying bacteria from reservoir sediments and utilize different electron donors for isolates to improve nitrate removal efficiency. Using traditional enrichment approach, one purified anaerobic bacterium (Y12) capable of NO-3-N removal from sediments was obtained. The species identity of Y12 was determined via 16S rRNA gene sequence analysis to be Acinetobacter. In this work, the fastest denitrification rates were observed with ferrous iron as electron donor. And, slightly slower rates were observed with hydrogen and sodium sulfide as electron donors. However, when used hydrogen gas, ferrous iron and sodium sulfide as electron donors, C/N ratios had little effect on autotrophic denitrification rate at the initial C/N ratio from 1.5 to 9.0. Meanwhile, when made use of hydrogen gas, ferrous iron and sodium sulfide as electron donors, a maximum nitrate removal ratio of 100.00%, 91.43% and 87.99% at the temperature of 30 ℃, respectively. Moreover, maximum denitrification activity was observed at pH 6.0-7.0. |
| Key words: eutrophication electron donor nitrate Acinetobacter sp autotrophic denitrification |
| DOI：10.11916/j.issn.1005-9113.2016.01.013 |
| Clc Number:X703.1 |
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