| Related citation: | Kai Wang,Siqi Li,Xiquan Cheng,Yingjie Zhang,Peisheng Yan,Jun Ma.Potential of Novel Halotolerant and Psychrotrophic Strain of Planococcus sp. for Water Treatment[J].Journal of Harbin Institute Of Technology(New Series),2018,25(5):24-32.DOI:10.11916/j.issn.1005-9113.17129. |
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| Author Name | Affiliation | | Kai Wang | School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209,Shandong, China
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China | | Siqi Li | School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209,Shandong, China | | Xiquan Cheng | School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209,Shandong, China | | Yingjie Zhang | School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209,Shandong, China | | Peisheng Yan | School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209,Shandong, China | | Jun Ma | School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China |
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| Abstract: |
| To screen multifunctional bacteria for water treatment, a chemical oxygen demand (COD) degradation and flocculation strain S2A15 was obtained from the offshore sewage outfall at Weihai International Beach. Based on the phylogenetic characteristics, a novel strain was identified as a Planococcus species. Strain S2A15 was determined to have the ability of flocculation and COD degradation. A series of experiments showed that the strain S2A15 could be used for the treatment of four types of wastewater, including domestic wastewater (400 mg/L and 800 mg/L) and high salt domestic wastewater (400 mg/L and 800 mg/L). Among them, the best effect was exerted by the strain that reduced by 76.9% in domestic wastewater with 400 mg/L COD. The flocculation ratio reached 60.19%. The optimal treatment conditions are also discussed. We confirmed that the strain S2A15 had salt tolerance and low temperature resistance. The best growth of S2A15 at salt concentration of 6% and further confirmed that the strain could degrade COD at a low temperature. |
| Key words: COD flocculation wastewater treatment Planococcus sp. salt-tolerant bacteria |
| DOI：10.11916/j.issn.1005-9113.17129 |
| Clc Number:X56 |
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| Descriptions in Chinese: |
| 一株新耐盐耐低温菌的鉴定及其在废水处理中的应用 王 凯1，2，李斯琪1，程喜全1，张瑛洁1，闫培生1，马 军2 （1. 哈尔滨工业大学 海洋科学与技术学院，威海 264209，山东； 2. 哈尔滨工业大学 市政与环境工程学院，哈尔滨 150090） 创新点说明：发现一株新菌，耐盐耐低温，既具有絮凝能力又可降解COD，其应用范围更广且功能更加多样，以期抵抗复杂多变的实际环境。 研究目的： 筛选出一株耐受盐度、温度范围更广范的多功能型水处理功能菌，以期为淡水或海水净化技术提供一种选择。 研究方法： 从山东威海金海湾排污口分离出一株橘黄色细菌，该菌株可用于处理无盐条件下的模拟废水（COD浓度分别为400和800 mg/L）和高盐条件下的模拟废水（COD分别为400和800 mg/L），并且探究该菌株在不同温度下处理以上四种模拟废水的能力。通过搅拌器确定了该菌株为絮凝剂产生菌，并通过一系列单因素实验确定了最佳絮凝条件及絮凝剂的分布。 结果： 1)该菌株S2A15在与模式菌株Planococcus maritimus DSM 17275 的相似度达98.8%。 2)该菌株S2A15在盐度为0-12%的条件下生长良好。 3)该菌株S2A15可在4℃-28℃的条件下生长。 4)从温度看，S2A15在28℃时COD降解能力最高；从处理废水浓度看，S2A15处理400 mg/L COD废水时几乎优于处理800 mg/L COD废水；从含盐度看，S2A15在无盐的条件下对COD的降解率几乎优于含盐废水。该菌株S2A15在28 ℃，400 mg/L COD的含盐模拟废水降解COD的效果最好，达到76.90%。 5)最佳的絮凝条件为：3 mL的10%氯化钙，2 mL的絮凝剂，pH为9，处理浓度为1 mg/L的高岭土悬液，其絮凝率能达到90%以上。 6)微生物絮凝剂主要分布在发酵液中。 结论： 1)经鉴定该菌株S2A15为动性球菌属。 2)菌株S2A15为耐盐且耐低温菌株。 3)在常温或低温，含盐或无盐条件下，菌株S2A15均有降解COD的能力。 4)该微生物絮凝是由菌株S2A15产生并分泌到细胞外具有絮凝活性的代谢产物，并且具有热稳定性。 关键词： COD；絮凝；废水处理；动性球菌属；耐盐菌株 |
