| Related citation: | Kai Wang,Xujiao Ma,Siqi Li,Xiquan Cheng,Yingjie Zhang,Jun Ma.Screening of Seawater Compound Bioflocculant Producing Bacterial Strains and Optimization of Their Culture Medium[J].Journal of Harbin Institute Of Technology(New Series),2022,29(3):1-12.DOI:10.11916/j.issn.1005-9113.21002. |
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| Author Name | Affiliation | | Kai Wang | School of Marine Science and Technology, Harbin Institute of Technology Weihai, Weihai 264209, China
School of Environment, Harbin Institute of Technology, Harbin 150090, China | | Xujiao Ma | School of Marine Science and Technology, Harbin Institute of Technology Weihai, Weihai 264209, China | | Siqi Li | School of Marine Science and Technology, Harbin Institute of Technology Weihai, Weihai 264209, China | | Xiquan Cheng | School of Marine Science and Technology, Harbin Institute of Technology Weihai, Weihai 264209, China | | Yingjie Zhang | School of Marine Science and Technology, Harbin Institute of Technology Weihai, Weihai 264209, China | | Jun Ma | School of Environment, Harbin Institute of Technology, Harbin 150090, China |
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| Abstract: |
| Compared with single bioflocculant, compound bioflocculant (CBF) normally has more stable and higher flocculation efficiencies. In order to produce a new compound bioflocculant, four bacterial strains with relative high rate of flocculation were selected from the coastal zone sediment, all of which were determined as Pseudomonas sp. by 16S rRNA sequencing. Through compounding, the ideal proportion of compound bioflocculant consisting of two bacterial strains (F5∶M5) was determined as 1∶2, resulting in 6% increase in terms of flocculation rate. The significant fermentation factors in culture media and their limitations were investigated via single factor experiments and Plackett-Burman design, obtaining four key variables, MgSO4, (NH4)2SO4, sucrose and peptone, which were further optimized by response surface methodology (RSM) for their optimal concentrations. Finally, the ideal culture medium was determined, containing (NH4)2SO4 0.523 g, MgSO4 3.7 g, sucrose 12.82 g, K2HPO4 4 g, KH2PO4 3 g, NaCl 33 g, and deionized water 1 L. The flocculation rate of experimental data (88.92) agreed well with the model prediction, which was 16.60 higher than original rate. |
| Key words: compound bioflocculant Plackett-Burman design response surface methodology |
| DOI：10.11916/j.issn.1005-9113.21002 |
| Clc Number:X56 |
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| Descriptions in Chinese: |
| 海洋复合型生物絮凝剂产生菌的筛选及培养基优化 王凯1,2，马旭蛟1，李斯琪1，程喜全1，张瑛洁1，马军2 (1. 哈尔滨工业大学, 海洋科学与技术学院, 山东 威海264209 ; 2. 哈尔滨工业大学, 环境学院, 哈尔滨 150090) 摘要： 与单一生物絮凝剂相比，复合型生物絮凝剂（CBF）通常具有更稳定、更高的絮凝效率。为制备新型复合生物絮凝剂，从海岸带沉积物中筛选出4株絮凝率较高的细菌，经16S rDNA测序鉴定均为假单胞菌。通过复配，确定了由其中两株菌（F5:M5）组成的复合生物絮凝剂的理想配比为1:2，絮凝率提高6%。通过单因素试验和Plackett-Burman设计，对发酵过程中培养基成分的主要影响因子及其限值进行研究，得到四个关键变量MgSO4、(NH4)2SO4、蔗糖和蛋白胨，并用响应面法（RSM）对其进行优化。最终确定优化的培养基为（NH4）2SO4 0.523g、MgSO4 3.7g、蔗糖12.82g、K2HPO4 4g、KH2PO4 3g、NaCl 33g、去离子水1L，絮凝率（88.92%）与模型预测值吻合较好，比原絮凝率提高16.60%。 关键词：复合型生物絮凝剂；Plackett-Burman设计；响应面法 |
