| 引用本文: | 刘枫,李建政,昌盛,陈忠林.产氢产乙酸互营共培养体7-m-2a的生长代谢特性[J].哈尔滨工业大学学报,2010,42(6):890.DOI:10.11918/j.issn.0367-6234.2010.06.012 |
| LIU Feng,LI Jian-zheng,CHANG Sheng,CHEN Zhong-lin.Physioecologic and metabolic products characteristics of a new isolated syntrophic acetogenesis coculture 7-m-2a[J].Journal of Harbin Institute of Technology,2010,42(6):890.DOI:10.11918/j.issn.0367-6234.2010.06.012 |
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| 摘要: |
| 为了对产氢产乙酸互营共培养体的扩大培养和应用奠定基础,采用静态培养实验,考察了碳源、氮源、温度和pH等因素对前期分离得到的新型共培养体7-m-2a的生长代谢特性的影响.结果表明,产氢产乙酸互营共培养体7-m-2a对丙酸、丁酸和苯甲酸都具有很高的转化率,其最适宜碳源为丁酸,不易利用葡萄糖和蔗糖;以蛋白胨和酵母粉混合物为氮源时,其生长代谢活动最旺盛,适宜的pH和温度分别为8.0和45℃. |
| 关键词: 厌氧消化 产氢产乙酸菌 互营共培养体 生理生态特性 |
| DOI：10.11918/j.issn.0367-6234.2010.06.012 |
| 分类号:X703 |
| 基金项目:哈尔滨市科技创新人才研究专项资金项目(2009RFXXS004) |
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| Physioecologic and metabolic products characteristics of a new isolated syntrophic acetogenesis coculture 7-m-2a |
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LIU Feng, LI Jian-zheng, CHANG Sheng, CHEN Zhong-lin
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State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology, Harbin 150090,China
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| Abstract: |
| In order to culture the syntrophic acetogenesis coculture in large scale and make it be used in fullscale application,the effect of carbon source,nitrogen source,temperature and pH on the activity of a new isolated syntrophic acetogenesis cocluture 7-m-2a was investigated in this study. The result of batch cultivation experiments shows that the syntrophic acetogenesis coculture 7-m-2a can utilize propionate,butyrate and benzoate as carbon source,and the most appropriate substrate is butyrate,while it almost can not degrade glucose and sucrose. The metabolic activity is the most active when using the mixture of tryptone and yeast as nitrogen source. The suitable cultivation temperature and pH are 45 ℃ and 8. 0. |
| Key words: anaerobic digestion syntrophic acetogenic bacteria syntrophic cocluture physioecologic characteristics |
