引用本文: | 王哲,魏利,马放,赵光,张斯.苯胺废水SBR工艺生物强化处理效能[J].哈尔滨工业大学学报,2010,42(6):949.DOI:10.11918/j.issn.0367-6234.2010.06.026 |
| WANG Zhe,WEI Li,MA Fang,ZHAO Guang,ZHANG Si.Treatment effectiveness of bioaugmentation on aniline wastewater with SBR[J].Journal of Harbin Institute of Technology,2010,42(6):949.DOI:10.11918/j.issn.0367-6234.2010.06.026 |
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摘要: |
应用生物强化技术,将实验室筛选出的高效苯胺降解菌投加到SBR反应器中,考察其经生物强化后对苯胺的去除效果,COD、氨氮、亚硝氮、硝氮的变化及微生物群落的演替规律.结果表明,生物强化系统可有效实现对苯胺的降解,系统达到稳定运行后,苯胺降解率为100%,COD的去除率为93%左右,氨氮的浓度随苯胺降解而升高.因水力滞留时间较短,并未驯化出亚硝化、硝化细菌.扫描电镜照片可以看出,系统稳定运行后,苯胺降解菌遍布于活性污泥上.此外,由PCR-DGGE图谱及相似性矩阵图可见,经16周期的运行,微生物群落种类、数量产生了明显的差异性,系统内微生物优势种群得到强化. |
关键词: 生物强化 苯胺降解 群落演替 PCR-DGGE |
DOI:10.11918/j.issn.0367-6234.2010.06.026 |
分类号:X703.1 |
基金项目:国家自然科学基金资助项目(50908063);哈工大优秀青年教师基金资助项目(NQQQ92324547) |
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Treatment effectiveness of bioaugmentation on aniline wastewater with SBR |
WANG Zhe, WEI Li, MA Fang, ZHAO Guang, ZHANG Si
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State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology, Harbin 150090,China
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Abstract: |
Aniline-degrading bacteria conserved by laboratory were tested in Sequencing Batch Reactor(SBR) to investigate the capability of aniline removal,the variation of interrelated index such as COD,NH 4+ ,NO 2-and NO 3-,and the community succession of microorganism. The results showed that SBR started up quickly and degraded aniline effectively. The removal efficiency of aniline reached 100% and the removal efficiency of COD was about 93% when the system reached steady running. The photographs screened by scanning electron microscope(SEM) indicated that the aniline-degrading bacteria spread over the activated sludge. It was found from the PCR-DGGE map and the similarity matrix that the species and the amounts of microorganism changed greatly,and dominant species in the system were strengthened. |
Key words: bioaugmentation aniline-degradation community succession PCR-DGGE |