| 引用本文: | 常玉广,马放,王博,王弘宇.高效絮凝菌的鉴定及絮凝特性研究[J].哈尔滨工业大学学报,2010,42(10):1609.DOI:10.11918/j.issn.0367-6234.2010.10.020 |
| CHANG Yu-guang,MA Fang,WANG Bo,WANG Hong-yu.Identification and flocculent analysis on high efficient flocculation producing bacteria[J].Journal of Harbin Institute of Technology,2010,42(10):1609.DOI:10.11918/j.issn.0367-6234.2010.10.020 |
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| 摘要: |
| 从含油废水曝气池活性污泥中筛选6株具有絮凝特性的菌株,命名为:F1、F2、F3、F4、F5、F6.为了研究絮凝菌的分类及絮凝特性,采用16SrDNA序列方法对6株絮凝菌进行鉴定分类;利用紫外扫描光谱与红外光谱扫描光谱分析絮凝活性成分.鉴定结果表明:6株絮凝菌分别与芽孢杆菌属、农杆菌属、库克菌属聚为一类,特征基本一致,具有99%同源性;絮凝测定结果显示,絮凝率在69%以上,其中F2、F3、F5、F6的絮凝率高于80%.将上述4株絮凝菌进行双菌混合培养试验,结果显示:F2和F6组合絮凝活性最佳,均优于各单菌,絮凝剂的有效成分为多糖类物质. |
| 关键词: 絮凝 16SrDNA序列 系统发育分析 多糖 |
| DOI：10.11918/j.issn.0367-6234.2010.10.020 |
| 分类号:X703.5 |
| 基金项目:国家重点基础研究发展计划项目(2004CB418505);中国博士后科学基金资助项目(20070420671) |
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| Identification and flocculent analysis on high efficient flocculation producing bacteria |
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CHANG Yu-guang1,2, MA Fang1, WANG Bo1, WANG Hong-yu1
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1.School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China;2.School Biochemical and Environmental Engineering,Nanjing Xiaozhuang University,Nanjing 211171,China)
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| Abstract: |
| Six strains with flocculent characteristic have been screened from oil-field wastewater,which are named as F1,F2,F3,F4,F5 and F6.They were identified and classified by 16SrDNA sequence.The flocculation species were analyzed through UV and IR scanning spectrophotometry.It is showed that the six strains belong to the same species with Balillus sp.,Agrobacterlum sp.,and Kocurla sp.and they have 99% homogeneous identity.The results of flocculent efficiency show that the flocculent efficiency of the six strains is more than 69%,among which the flocculent efficiencies of F2,F3,F5 and F6 are more than 80%.Mixed cultures were conducted pairwise among F2,F3,F5 and F6,and the results show that the combination of F2 and F6 strains exhibits the best flocculating efficiency and it is higher than that of any pure strain. |
| Key words: flocculation 16SrDNA sequence phylogenetic analysis polysaccharide |
