| 摘要: |
| 为了定量分析水中光合细菌(PSB)菌体产量,为光合细菌污水资源化提供方法依据,采用HPLC法分析CoQ10的质量,进而测得水中PSB的菌体产量.实验结果表明, 色谱条件为: 色谱柱为 SphherisorbC18 (10 cm×4.6 mm ID) 柱;甲醇与无水乙醇为流动相,体积比9∶1;流速为1 mL／min;柱温35 ℃;紫外检测波长275 nm;进样量15 μL;柱压16.4 MPa.先用丙酮提取5 h后挥掉全部丙酮, 再用无水乙醇溶解,这一提取方法对CoQ10的相对提取率最高.加外标检测法平均回收率为99.13％,相对标准偏差为0.81％ (n=5).不同培养条件及生长时期的PSB内CoQ10的含量十分稳定.废水中杂质、杂菌等干扰因素均不影响CoQ10的检测结果.PSB质量浓度为(Y+37.487)／25.431 g/L,其中Y为峰面积(V·s).该方法简便易行, 结果准确, 可用于水中PSB菌体含量的定量分析. |
| 关键词: HPLC PSB CoQ10 菌体产量 污水资源化 |
| DOI： |
| 分类号:Q936 |
| 文献标识码:A |
| 基金项目:国家自然科学基金重点项目( 50978072). |
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| HPLC quantification of photosynthetic bacteria in water |
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ZHAO Wei,ZHANG Guang-ming
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(School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090 Harbin,China,gmgwen@gmail.com)
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| Abstract: |
| To test biomass of photosynthetic bacteria (PSB) in water, and to provide a way of PSB sewage reclamation, this paper adopted HPLC to analyze the concentration of CoQ10and thus obtained the concentration of PSB in water. The results show that the chromatographic condition is as follows: The chromatographic column is SphherisorbC18 (10 cm×4.6 mm ID); methanol-absolute alcohol (9∶1) as mobile phase at a flow rate of 1 mL／min; column temperature 35 ℃; Wave Length of UV detector 275 nm; sample size 15 μL; column pressure 16.4 MPa. By extracting 5 h with acetone, volatilizing acetone completely and dissolving it with absolute alcohol, this method may obtain the maximum relative extraction of CoQ10. The average recovery ratio of external reference method is 99.13％ and RSD 0.81％. The highest radiance-extraction is acetone extraction 5 h before evaporating completely and then solving in ethanol. The concentration of CoQ10 in PSB in various cultural conditions and growing phases is quite stable. The impurities or microbes in wastewater do not affect the testing result. The concentration of PSB is (Y+37.487)／25.431 g·L-1, Y is peak area(V·s). This method is simple and accurate, and can be used for quantitative analysis of PSB in water. |
| Key words: HPLC PSB CoQ10 biomass sewage reclamation |
