| 引用本文: | 张光明,郎朗,李钰,马军.E2弱化双酚A雌激素活性机制[J].哈尔滨工业大学学报,2012,44(4):28.DOI:10.11918/j.issn.0367-6234.2012.04.006 |
| ZHANG Guang ming,LANG Lang,LI Yu,MA Jun.Mechanisms of E2 weakening effects on BPA’s estrogenicity[J].Journal of Harbin Institute of Technology,2012,44(4):28.DOI:10.11918/j.issn.0367-6234.2012.04.006 |
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| 摘要: |
| 前期研究发现天然雌激素E2可以弱化一种常见雌激素类污染物双酚A的活性,为探讨这一现象的机制,利用人体乳腺癌细胞MCF 7,从细胞周期、雌激素受体途径、MAPKs信号途径进行实验．结果表明:E2可诱导DNA合成期细胞显著增高,抑制受体蛋白表达,提高ERK蛋白的表达,进而激活多种转录因子;双酚A显著抑制受体蛋白表达,对MAPKs信号通路基本没有影响,可同时提高DNA分裂期与间歇期细胞比例;两者联合后活化了受体途径和ERK途径,促进C fos的转录表达,激活cyclin D1蛋白,增加S期及G2期细胞比例．E2弱化双酚A的可能机制为C myc蛋白表达下降． |
| 关键词: 雌二醇 双酚A 雌激素活性 C myc蛋白表达 细胞周期 |
| DOI：10.11918/j.issn.0367-6234.2012.04.006 |
| 分类号:X7031 |
| 基金项目:国家高技术研究发展计划项目(2007AA06Z339);水体污染控制与治理科技重大专项课题(2009ZX07424 005) |
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| Mechanisms of E2 weakening effects on BPA’s estrogenicity |
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ZHANG Guangming1,2, LANG Lang1,3, LI Yu4, MA Jun1
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1.School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090 Harbin,China;2.School of Environment and Natural Resources, Renmin Univeristy of China, 100872 Beijing, China;3.Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, 150028 Harbin,China;4.School of Science, Harbin Institute of Technology, 150001 Harbin,China
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| Abstract: |
| Previous study showed that natural estrin, 17 Estradiol (E2), could weaken the estrogenicity of Bisphenol A (BPA), a common estrin in water resources. In order to learn the potential mechanisms, experiments were performed with human breast cancer cell MCF 7 on the cell cycle, estrogen receptor pathway and MAPKs pathway. Results showed that E2 worked through estrogen receptors and ERK pathway, and activated C myc gene, C fos gene, and cyclin D1 protein, and thus increased the S section of the cell cycle. BPA worked mainly through estrogen receptors by activating cyclin D1 protein, and increased the S and G2 sections of the cell cycle. The mixture of BPA and E2 worked through both pathways, activated both C fos and cyclin D1, and increased both S and G2 sections of the cell cycle. The main mechanism of E2’s weakening on BPA’s estrogenicity might be the decreased expression of C myc protein. |
| Key words: 17-Estradiol BPA estrogenicity C myc protein expression cell cycle |
