| 引用本文: | 迟国达,王鹏,徐伟,黄臣勇.阴离子交换树脂分离提取发酵液中的乳酸[J].哈尔滨工业大学学报,2012,44(4):39.DOI:10.11918/j.issn.0367-6234.2012.04.008 |
| CHI Guo da,WANG Peng,XU Wei,HUANG Chen yong.Extraction of lactic acid from fermentation broth with anion exchange resin[J].Journal of Harbin Institute of Technology,2012,44(4):39.DOI:10.11918/j.issn.0367-6234.2012.04.008 |
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| 摘要: |
| 为提高乳酸产品收率和纯度,实验筛选得到国产315型阴离子交换树脂,用于分离提取发酵液中乳酸,并研究其对乳酸、乙酸、丙酮酸及柠檬酸的吸附等温线、吸附动力学和动态吸附及洗脱效果．吸附等温线结果表明,315型树脂对乳酸等有机酸的吸附等温线符合Freundlich方程,特征参数n>1,属于优惠吸附．吸附动力学研究表明,液膜扩散是吸附过程的主要控制步骤,符合Boyd 液膜扩散模型．动态吸附及洗脱实验结果表明,以15 BV／h、pH 188上柱吸附,再用1 BV／h的去离子水洗脱,可实现发酵液中乳酸的良好分离． |
| 关键词: 阴离子交换树脂 乳酸 提取 吸附等温线 吸附动力学 |
| DOI：10.11918/j.issn.0367-6234.2012.04.008 |
| 分类号:TQ4207 |
| 基金项目:国家自然科学基金资助项目(50778053) |
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| Extraction of lactic acid from fermentation broth with anion exchange resin |
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CHI Guoda1, WANG Peng1, XU Wei1,2, HUANG Chenyong1
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1.State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 150090 Harbin, China;2.School of Food Engineering, Harbin University of Commerce, 150076 Harbin, China
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| Abstract: |
| The domestic anion exchange resin 315 was selected to separate lactic acid from the fermentation broth. Adsorption isotherm, kinetics, effect of dynamic adsorption and elution on lactic acid, acetic acid, pyruvic acid and citric acid were studied. Adsorption isotherm results showed that the Freundlich isotherm equation agreed well with the adsorption data of organic acids on anion exchange resin 315, and the characteristic parameter of this equation n>1. It displayed that the adsorption process belonged to favorable adsorption. Besides, the adsorption kinetics of organic acids on anion exchange resin 315 was mainly controlled by liquid film diffusion, and it accorded well with the Boyd liquid film diffusion model. The optimum dynamic adsorption and elution conditions were determined: adsorption flow rate, 15 BV／h; pH 188; eluent, deionized water; elution flow rate, 1 BV／h. Under these conditions, the lactic could be well separated from the fermentation broth to achieve the purpose of increasing the yield of lactic acid products and improving product purity. |
| Key words: anion exchange resin lactic acid extraction adsorption isotherm adsorption kinetics |
