| 引用本文: | 郭长虹,刘庆国,刘佳莉,束永俊,吕月萍,余建平.aadA叶绿体转化烟草对根际微土壤生物的影响[J].哈尔滨工业大学学报,2012,44(4):141.DOI:10.11918/j.issn.0367-6234.2012.04.028 |
| GUO Chang hong,LIU Qing guo,LIU Jia li,SHU Yong jun,L Yue ping,YU Jian ping.Effects of [WT4HX]aadA[WT4HZ] transplastomic tobacco on rhizosphere microbial communities in soil[J].Journal of Harbin Institute of Technology,2012,44(4):141.DOI:10.11918/j.issn.0367-6234.2012.04.028 |
|
| 摘要: |
| 为研究叶绿体转基因烟草对土壤根际微生物的影响,以aadA叶绿体转基因烟草和对照烟草为实验材料,对苗期、花期、成熟期烟草根际细菌、放线菌及真菌的菌落数进行统计,利用Biolog法分析微生物群落功能多样性．结果表明:在相同生育期,aadA叶绿体转基因烟草与对照烟草相比,根际土壤细菌、放线菌及真菌数量均无显著差异;根际土壤微生物群落的代谢相似,转基因烟草对微生物群落功能的影响不显著．aadA叶绿体转基因烟草没有对土壤微生物数量和功能多样性产生显著的影响． |
| 关键词: 叶绿体转化烟草 根际微生物 Biolog法 aadA基因 |
| DOI：10.11918/j.issn.0367-6234.2012.04.028 |
| 分类号:S-3 |
| 基金项目:家自然科学基金资助项目 (30970552)；黑龙江省高校科技创新团队研究计划项目(2010TD10);哈尔滨师范大学科技创新团队研究计划项目(KJTD-2011-2) |
|
| Effects of [WT4HX]aadA[WT4HZ] transplastomic tobacco on rhizosphere microbial communities in soil |
|
GUO Changhong, LIU Qingguo, LIU Jiali, SHU Yongjun, L Yueping, YU Jianping
|
|
Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and
Technology, Harbin Normal University, 150025 Harbin,China
|
| Abstract: |
| To study the effects of aadA transplastomic tobacco (TA) on soil microbial communities, the quantity of bacteria, actinomycetes, and fungi was investigated at seedling stage, flowering stage, and senescing stage by plate cultivation of microorganism, and the functional diversity of rhizosphere microorganisms was detected by Biolog Ecoplate method. The results showed that there was no significant difference on the populations of bacteria, actinomycetes, and fungi between aadA transplastomic tobacco and non transgenic tobacco at same growing stage. The Biolog analysis indicated that the metabolism of rhizosphere microbial community were similar, aadA transplastomic tobacco didn’t affect the microbial community. In summary, aadA transplastomic tobacco had no significant effects on quantity and functional diversity of rhizosphere microorganisms in soil during one year growth. |
| Key words: transplastpmic tobacco rhizosphere microbial Biolog aadA gene |
