| 引用本文: | 曾涛涛,李冬,邱文新,曾辉平,刘涛,张杰.低温厌氧氨氧化生物滤池群落结构分析[J].哈尔滨工业大学学报,2012,44(6):6.DOI:10.11918/j.issn.0367-6234.2012.06.002 |
| ZENG Tao-tao,LI Dong,QIU Wen-xin,ZENG Hui-ping,LIU Tao,ZHANG Jie.Community structure analysis of anaerobic ammonium oxidation (ANAMMOX) biofilter at low temperature[J].Journal of Harbin Institute of Technology,2012,44(6):6.DOI:10.11918/j.issn.0367-6234.2012.06.002 |
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| 摘要: |
| 为了解低温(150～165 ℃)稳定运行的厌氧氨氧化生物滤池微生物特征,利用扫描电镜(SEM)、变性梯度凝胶电泳技术(DGGE)和克隆测序等方法,对低温稳定运行的两个上流式厌氧氨氧化生物滤池进行微生物群落结构分析．SEM结果显示,陶粒填料反应器(B1)内丝状菌较多,球形细菌分布密度较低,而火山岩填料反应器(B2)没有发现丝状菌,球形细菌分布密集．DGGE结果表明,B1与B2反应器微生物种类有所差别,种群相似性为681％,B2反应器内细菌丰度更高,但两反应器内厌氧氨氧化细菌种类相同．通过细菌及ANAMMOX菌16S rRNA克隆测序,鉴定反应器内厌氧氨氧化细菌为Candidatus Kuenenia stuttgartiensis．采用火山岩填料生物滤池反应器形式,并富集Candidatus Kuenenia stuttgartiensis,有助于厌氧氨氧化工艺在较低温度(150～165 ℃)条件下稳定运行和推广应用． |
| 关键词: 厌氧氨氧化 变性梯度凝胶电泳 生物滤池 低温 |
| DOI：10.11918/j.issn.0367-6234.2012.06.002 |
| 分类号:X172 |
| 基金项目:教育部跨世纪优秀人才支持计划（NCET-10-0008);城市水资源与水环境国这有重点实验室开放基金（QAK201005);国家重大科技专项水专项（2012ZX 07202-005) |
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| Community structure analysis of anaerobic ammonium oxidation (ANAMMOX) biofilter at low temperature |
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ZENG Tao-tao1, LI Dong2, QIU Wen-xin2, ZENG Hui-ping2, LIU Tao1, ZHANG Jie1,3
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1.State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology, 150090 Harbin, China;2.Key Laboratory of
Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, 100124 Beijing, China;3.Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, 100124 Beijing, China
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| Abstract: |
| To characterize the microbial community in anaerobic ammonia oxidation (ANAMMOX) biofilter and to apply this technology, techniques of scanning electron microscopy (SEM), denaturing gradient gel electrophoresis (DGGE), cloning and sequencing were utilized to study the community structures in two up-flow ANAMMOX biofilters, which were operated stably at low temperature (150-165 ℃). The results of SEM showed that the filamentous bacteria were just found within ceramic packing B1 biofilter. Spherical bacteria had more densely distribution in volcanic packing B2 biofilter than that in B1 biofilter. Bacterial DGGE results showed that there were some differences between B1 and B2 microbial species and the population similarity was 681％, and B2 reactor had higher bacteria richness. However, the type of anammox bacteria existence in two reactors was the same. The sequences of bacteria and ANAMMOX 16S rRNA identification revealed that Candidatus Kuenenia stuttgartiensis occurred in two ANAMMOX bioreactors. It was helpful to apply ANAMMOX technology at low temperature (150-165 ℃) through Candidatus Kuenenia stuttgartiensis enrichment, with the form of biofilter reactor filled with volcanic rocks. |
| Key words: anaerobic ammonium oxidation denaturing gradient gel electrophoresis (DGGE) biofilter low temperature |
