| 引用本文: | 刘畅,杨基先,李昂,李旭,马放,陆建.复合诱变选育高效生物破乳剂产生菌及其特性[J].哈尔滨工业大学学报,2012,44(6):24.DOI:10.11918/j.issn.0367-6234.2012.06.006 |
| LIU Chang,YANG Ji-xian,LI Ang,LI Xu,MA Fang,LU Jian.Breeding and characteristics of the high efficient biodemulsifier-producing bacteria by mutagenesis[J].Journal of Harbin Institute of Technology,2012,44(6):24.DOI:10.11918/j.issn.0367-6234.2012.06.006 |
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| 摘要: |
| 为提高破乳性能,以一株生物破乳剂产生菌Bacillus mojavensis XH1为出发菌株,进行紫外和亚硝基胍复合诱变处理,经过筛选分离和连续传代得到一株遗传稳定性较好的高效生物破乳剂产生菌突变株XN5．在碳源为10 g／L葡萄糖和4％ (体积分数)液体石蜡的混合碳源、氮源为40 g／L NH4Cl与10 g／L酵母膏的混合氮源、温度30 ℃、初始pH=65、摇床转数为140 r／min的培养条件下,培养24 h后,突变菌株的12 h和24 h破乳率分别为9417％和9867％,比原始菌株XH1提高了6481％和1512％． |
| 关键词: 生物破乳剂 紫外 亚硝基胍(NTG) 诱变 |
| DOI：10.11918/j.issn.0367-6234.2012.06.006 |
| 分类号:X172; TE991 |
| 基金项目:国家高技术研究发展计划项目(2009AA062906); 国家创新研究群体科学基金资助项目(51121062) |
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| Breeding and characteristics of the high efficient biodemulsifier-producing bacteria by mutagenesis |
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LIU Chang1, YANG Ji-xian1, LI Ang1, LI Xu1, MA Fang1, LU Jian2
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1.State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 150090 Harbin, China;2.Science and Technology Bureau, China Yixing Industrial Park for Environmental Science and Technology,
214200 Yixing， Jiangsu, China
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| Abstract: |
| To improve demulsifying property, the original biodemulsifier-producing bacterium, Bacillus mojavensis XH1, was mutated and bred by the combined treatment method using UV irradiation and nitrosoguanidine (NTG). A high efficient biodemulsifier-producing mutant XN5 with the better stability was obtained after continuous passages. The optimal cultural conditions were as follows: the mixture of 10 g／L glucose and 4 ％ liquid paraffin is the carbon source, the mixture of 40 g／L NH4Cl and 10 g／L yeast extract is nitrogen source, pH=65, the rotating rate is 140 r／min and the incubated time is 24 h. The 12 h and 24 h demulsification ratios of the mutant XN5 were 9417％ and 9867％, which were 6481％ and 1512％ higher than that of the original strain XH1, respectively. |
| Key words: biodemulsifier UV nitrosoguanidine (NTG) mutagenesis |
