| 引用本文: | 马放,蔡蕊,李昂,崔迪,王继华,庞长泷,邱天,杨基先.微生物非培养技术在环境污染控制领域的应用[J].哈尔滨工业大学学报,2012,44(8):31.DOI:10.11918/j.issn.0367-6234.2012.08.007 |
| MA Fang,CAI Rui,LI Ang,CUI Di,WANG Ji-hua,PANG Chang-long,QIU Tian,YANG Ji-xian.Application of microbial culture-independent technology in environment contamination control[J].Journal of Harbin Institute of Technology,2012,44(8):31.DOI:10.11918/j.issn.0367-6234.2012.08.007 |
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| 微生物非培养技术在环境污染控制领域的应用 |
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马放1,2, 蔡蕊3,2, 李昂1,2, 崔迪1,2, 王继华4, 庞长泷1,2, 邱天1,2, 杨基先1,2
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1.哈尔滨工业大学 城市水资源与水环境国家重点实验室, 150090 哈尔滨;2.哈尔滨工业大学 市政环境工程学院, 150090 哈尔滨;3.哈尔滨工业大学 城市水资源与水环境国家重点实验室, 150090 哈尔滨;4.哈尔滨师范大学 生命科学与技术学院， 150025 哈尔滨
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| 摘要: |
| 微生物非培养技术,如DNA指纹技术、焦磷酸测序、分子杂交技术、宏基因组学、宏转录组学、宏蛋白质组学、代谢组学等的飞跃发展和广泛应用,有助于快速、准确地鉴定自然界或人工生境中微生物种群,为揭示生物修复过程中微生物群落结构、功能及修复机理提供了重要方法,提高了生物修复技术的检测效能．主要介绍分子生物学及“组学”等微生物非培养技术在解析污染区域中微生物群落结构、功能及动力学等方面的应用． |
| 关键词: 生物修复 宏基因组学 宏蛋白质组学 代谢组学 |
| DOI：10.11918/j.issn.0367-6234.2012.08.007 |
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| 基金项目:国家自然科学基金资助项目(51108120; 51178139); 第46批中国博士后科学基金资助项目(20090460901); 第4批中国博士后科学基金特别资助项目(201104430). |
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| Application of microbial culture-independent technology in environment contamination control |
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MA Fang1,2, CAI Rui1,2, LI Ang1,2, CUI Di3,2, WANG Ji-hua4, PANG Chang-long3,2, QIU Tian3,2, YANG Ji-xian3,2
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1.State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 150090 Harbin, China;2.School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090 Harbin, China ;3.State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 150090 Harbin, China ;4.School of Life Science and Technology, Harbin Normal University, 150025 Harbin, China
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| Abstract: |
| Microbial culture-independent technology, such as DNA fingerprinting technology, pyrosequencing, molecular hybridization technology, metagenomics, metatranscriptomics, metaproteomics, metabolomics and so on, has developed rapidly and been applied extensively in many fields, which could assist in the identification of microbial species from natural or anthropogenic environment rapidly and accurately. The microbial culture-independent technology has provided crucial in-sights for revealing microbial community structures, functions and remediation mechanism during bioremediation process and improving the detection efficiency of bioremediation. This review is mainly focused on the advanced applications of molecular biology and microbial culture-independent technology of “-omics” in gauging microbial community structures, functions and dynamics at contaminated areas. |
| Key words: bioremediation metagenomics metatranscriptomics metabolomics |
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