| 引用本文: | 刘亚利,袁一星,李欣,詹技灵,康晓荣,董林沛.污泥发酵液对A2O脱氮除磷和微生物的影响[J].哈尔滨工业大学学报,2014,46(10):42.DOI:10.11918/j.issn.0367-6234.2014.10.007 |
| LIU Yali,YUAN Yixing,LI Xin,ZHAN Jiling,KANG Xiaorong,DONG Linpei.The effect of sludge fermentation liquid on nutrient removal performances and microbial community structure in A2O process[J].Journal of Harbin Institute of Technology,2014,46(10):42.DOI:10.11918/j.issn.0367-6234.2014.10.007 |
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| 摘要: |
| 为研究剩余污泥发酵液作碳源对微生物群落结构的影响,将发酵液与市政污水按流量比1∶35回用于厌氧-缺氧-好氧反应器,在室温下运行90 d．聚类分析表明,发酵液明显改变了微生物群落结构,5~30 d和45~90 d的微生物属于不同的聚集区; 微生物多样性分析表明,发酵液使Shannon-Wiener指数从2.6升高到3.1,系统运行稳定性增强; PCR-DGGE分析表明,发酵液对微生物群落具有一定的选择性,氨氧化菌Nitrosomonas sp.、硝化菌Betaproteobacteria和 Nitrospira sp.、反硝化菌Comamonas sp.和聚磷菌Gammaproteobacteria得到富集,TN和TP去除率从64.5％和52.4％提高到84.7％和94.3％． |
| 关键词: 剩余污泥 脱氮除磷 碳源 生物群落 聚合酶链式反应-变性梯度凝胶电泳技术 |
| DOI：10.11918/j.issn.0367-6234.2014.10.007 |
| 分类号:TU992.3 |
| 基金项目:国家高技术研究发展计划(863计划)资助项目 (2012AA063503-02). |
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| The effect of sludge fermentation liquid on nutrient removal performances and microbial community structure in A2O process |
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LIU Yali1, YUAN Yixing1, LI Xin1, ZHAN Jiling2, KANG Xiaorong1, DONG Linpei1
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(1.School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090 Harbin, China; 2.Dalian MEC Environmental Technology & Engineering Co., Ltd, 116000 Dalian, Liaoning, China)
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| Abstract: |
| To analyze the effect of sludge fermentation liquid, using as internal carbon source, on microbial community structure in anaerobic-anoxic-aerobic process, three-month-long operational experiment was conducted at flow ratio of fermentation liquid and domestic wastewater 1∶35 at room temperature. The clustering analysis indicated that the microbial community structure was changed significantly by fermentation liquid, and the microbes of 5-30 d and 45-90 d had quite different homology. The microbial diversity analysis demonstrated that the Shannon-Wiener index increased from 2.6 to 3.1, resulting in the enhancement of operational stability. Meanwhile, fermentation liquid appeared to be selective for ammonia-oxidizing bacteria Nitrosomonas sp., nitrifying bacteria Betaproteobacteria and Nitrospira sp., denitrifying bacteria Comamonas sp. and phosphorus-accumulating bacteria Gammaproteobacteria, which led to the TN and TP removal efficiency improved from 64.5％ and 52.4％ to 84.7％ and 94.3％, respectively. |
| Key words: waste activated sludge nutrient removal carbon source bacterial community PCR-DGGE |
