| 引用本文: | 刘丽华,马万里,刘丽艳,李一凡.短链氯化石蜡C10 (50.2% Cl)对斑马鱼胚胎的发育毒性[J].哈尔滨工业大学学报,2016,48(8):127.DOI:10.11918/j.issn.0367-6234.2016.08.021 |
| LIU Lihua,MA Wanli,LIU Liyan,LI Yifan.Study on developmental toxicity of short-chain chlorinated paraffins C10 (50.2% Cl) in zebrafish embryos[J].Journal of Harbin Institute of Technology,2016,48(8):127.DOI:10.11918/j.issn.0367-6234.2016.08.021 |
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| 摘要: |
| 为评价短链氯化石蜡对斑马鱼胚胎的发育毒性，以斑马鱼为模式生物，观察不同质量浓度的短链氯化石蜡C10 (50.2% Cl)暴露24，48，72和96 h后斑马鱼胚胎/幼鱼的死亡率、孵化率、畸形率和体长.结果表明，C10 (50.2% Cl)在斑马鱼的早期发育阶段可引起一系列非致死效应和致死效应.高质量浓度(1 000和10 000μg/L)短链氯化石蜡可导致96 h斑马鱼胚胎死亡率显著升高(达100%)，48 h抑制胚胎的孵化，并诱发一系列的发育畸形.C10 (50.2% Cl)在斑马鱼幼鱼中引起的畸形表型主要包括脊柱弯曲，卵黄畸形，心包囊肿，尾部畸形和鱼鳔发育缺陷.低质量浓度组和高质量浓度组C10 (50.2% Cl)均能够抑制斑马鱼幼鱼的生长速度，目前的环境质量浓度已具有一定程度的水生生态风险.
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| 关键词: 短链氯化石蜡 斑马鱼 胚胎 发育毒性 |
| DOI：10.11918/j.issn.0367-6234.2016.08.021 |
| 分类号:X171.5 |
| 文献标识码:A |
| 基金项目:城市水资源与水环境重点实验室(哈尔滨工业大学)自主课题(2013DX15) |
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| Study on developmental toxicity of short-chain chlorinated paraffins C10 (50.2% Cl) in zebrafish embryos |
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LIU Lihua, MA Wanli, LIU Liyan, LI Yifan
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（International Joint Research Center for Persistent Toxic Pollutants (IJRC-PTS), State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China）
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| Abstract: |
| A zebrafish (Danio rerio) model was applied to evaluate the developmental toxicity of short-chain chlorinated paraffins (SCCPs). Death rates, hatching rates, malformation rates and body length were observed after the zebrafish embryos were exposed to different concentrations of C10 (50.2% Cl) separately for 24, 48, 72 and 96 h. The results indicated that C10 (50.2% Cl) could exert lethal and sub-lethal effects on the early life stage of zebrafish. Higher concentrations of C10 (50.2% Cl) (1 000 and 10 000μg/L) could not only cause significant death rates increase to 100% after 96 h exposure and hating delay after 48 h exposure, but also induce a series of malformations, including spinal curvature, yolk deformity, pericardial edema, malformation of tail and uninflated swim bladder. Since the growth inhibition of juvenile zebrafish caused by C10 (50.2% Cl) on the exposure concentrations no matter high or low, SCCPs might be a risk to the aquatic ecology and fish development.
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| Key words: SCCPs zebrafish embryo developmental toxicity |
