| 引用本文: | 由阳,彭永臻,王淑莹,刘秀红.强化生物除磷系统胞内聚合物测定方法优化[J].哈尔滨工业大学学报,2010,42(2):207.DOI:10.11918/j.issn.0367-6234.2010.02.007 |
| YOU Yang,PENG Yong-zhen,WANG Shu-ying,LIU Xiu-hong.Optimization of intracellular polymer analysis for enhanced biological phosphorous removal system[J].Journal of Harbin Institute of Technology,2010,42(2):207.DOI:10.11918/j.issn.0367-6234.2010.02.007 |
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| 摘要: |
| 为优化强化生物除磷系统胞内PHA、糖原以及多聚磷酸盐颗粒的测定方法,采用色谱与化学分析相结合的方法进行研究.其中,PHA的测定采用气相色谱法,糖原的测定采用稀盐酸消解加葡萄糖试剂盒法,多聚磷酸盐采用过硫酸钾氧化和钼锑抗光度法,经过方法优化得出4%酸化甲醇消解20h是提高PHA回收量的最佳条件;糖原在0.6mol/LHCl条件下消解5h提取量最大,临床中的葡萄糖氧化酶法试剂盒可以应用到本实验中;在120℃下氧化30min条件下多聚磷酸盐颗粒可以最大回收.经过加标回收实验等对这3个方法进行精密度、准确度分析,结果显示,在这3个条件下的3种方法都具有很高的精密度或准确度,而且简单易行,适合实验室及生产实践中的应用. |
| 关键词: 强化生物除磷 聚磷 多聚脂肪酸 糖原 聚磷菌 |
| DOI：10.11918/j.issn.0367-6234.2010.02.007 |
| 分类号:X703 |
| 基金项目:国家自然科学基金海外青年学者合作项目(50628808);城市水资源与水环境国家重点实验室开放研究项目(QAK200802);北京市教委科技创新平台项目(PXM2008-014204-050843) |
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| Optimization of intracellular polymer analysis for enhanced biological phosphorous removal system |
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YOU Yang1, PENG Yong-zhen1,2, WANG Shu-ying2, LIU Xiu-hong1
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1.School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China;2.College of Environmental and Energy Engineering,Beijing University of Technology,Beijing 100124,China)
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| Abstract: |
| The combination of chromatogram and chemical analysis was adopted to optimize the methods selected to measure poly-P,glycogen and PHA intracellular polymer in EBPR system.PHA was measured by GC system,glycogen was degraded by dilute hydrochloric acid and analyzed by glucose commercial kit,and poly-P was oxidized by potassium persulfate and analyzed by molybdenum-antimony anti-spectrophotometric method.Testing of the proposed method for PHA analysis shows that 4% sulfuric acid concentration and degradation for 20 h are required for optimal PHB and PHV extraction,while 0.6 mol/L HCl and digestion for 5 h produce the highest recovery of glycogen,and the medical glucose kit can be used in this research.Oxidation for 30 min at 120 ℃ is recommended for the recovery of poly-P.The results of precision analysis and recovery test with standard addition of these methods show that they possess high precision and are convenient for laboratory research and practical application. |
| Key words: enhanced biological phosphorus removal system poly-phosphate polyhydroxyalkanoates glycogen phosphorous accumulating organisms |
