| 引用本文: | 谭东徽,曲媛媛,马放,周集体,袁晓东.Arthrobacter sp. W1苯酚降解特性及其羟化酶基因获取[J].哈尔滨工业大学学报,2010,42(12):1977.DOI:10.11918/j.issn.0367-6234.2010.12.027 |
| TAN Dong-hui,QU Yuan-yuan,MA Fang,ZHOU Ji-ti,YUAN Xiao-dong.Characteristics of Arthrobacter sp. W1 for phenol biodegradation and acquisition of phenol hydroxylase gene[J].Journal of Harbin Institute of Technology,2010,42(12):1977.DOI:10.11918/j.issn.0367-6234.2010.12.027 |
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| 摘要: |
| 为获得适应高盐环境的苯酚降解菌及其相关降解基因,从活性污泥中筛选得到一株耐盐苯酚降解菌W1.利用16S rRNA基因序列鉴定该菌株,并考察了其降解特性;同时,采用Tail-PCR方法对菌株苯酚羟化酶基因进行侧翼调取.结果表明:菌株W1为节杆菌(Arthrobacter sp.),能在质量分数为1%～10%的NaCl溶液中以苯酚为唯一碳源及能源生长,并能降解对甲基酚、水杨酸、对苯二酚等多种芳香化合物.在质量分数为5%的NaCl溶液中,菌株W1对质量浓度为1 000 mg.L-1的苯酚降解率高达90%以上.侧翼获取的基因全长约为6 kb,其中,编码苯酚羟化酶大亚基基因的全长序列与Alcaligenes sp.相应序列具有较高的同源性,约为93%. |
| 关键词: 苯酚 羟化酶 Tail-PCR 生物降解 耐盐菌 |
| DOI：10.11918/j.issn.0367-6234.2010.12.027 |
| 分类号:X172 |
| 基金项目:国家自然科学基金资助项目(51078054,20923006);城市水资源与水环境国家重点实验室开放基金(QAK201009) |
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| Characteristics of Arthrobacter sp. W1 for phenol biodegradation and acquisition of phenol hydroxylase gene |
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TAN Dong-hui1,2,3, QU Yuan-yuan1, MA Fang2, ZHOU Ji-ti1, YUAN Xiao-dong3
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1.Key Laboratory of Industrial Ecology and Environmental Engineering of Ministry of Education,School of Environmental Science and Technology,Dalian University of Technology,Dalian 116024,China;2.State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China;3.TaKaRa Biotechnology(Dalian) Co.,Ltd.,Dalian 116600,China
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| Abstract: |
| To obtain novel salt-tolerant phenol-degrading bacteria and amplify the corresponding genes,a bacterium named W1 was isolated from the active sludge samples.16S rRNA sequence analysis was used to identify the bacterium,and characteristics for phenol biodegradation were also studied.The 5’-and 3’-flanking regions of gene encoding the phenol hydroxylase were amplified from strain W1 by TAIL-PCR method.1wt%-10wt% was showed that strain W1 was identified as Arthrobacter sp.The strain was capable of growing in the medium with 1wt%-10wt% NaCl and utilizing phenol as the sole carbon and energy source.And it could also degrade some other aromatic compounds such as p-methylphenol,salicylic acid and p-hydroquinone,etc.When concentration of NaCl was about 5wt%,1 000 mg·L-1 phenol could be degraded more than 90% by strain W1.The complete gene cluster was about 6 kb,of which the gene encoding the large subunit of phenol hydroxylase exhibited the highest similarity about 93% with the corresponding gene of Alcaligenes sp. |
| Key words: phenol hydroxylase Tail-PCR biodegradation salt-tolerant bacteria |
