| 引用本文: | 王磊,黄松,黄丹曦,王旭东,吕永涛,贾世发.QCM-D研究BSA在不同改性PVDF超滤膜表面的吸附行为[J].哈尔滨工业大学学报,2016,48(8):78.DOI:10.11918/j.issn.0367-6234.2016.08.013 |
| WANG Lei,HUANG Song,HUANG Danxi,WANG Xudong,Lü Yongtao,JIA Shifa.Adsorption behaviors of BSA on different modified PVDF ultrafiltration membranes using QCM-D[J].Journal of Harbin Institute of Technology,2016,48(8):78.DOI:10.11918/j.issn.0367-6234.2016.08.013 |
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| 摘要: |
| 为进一步探究经不同改性添加剂(聚乙烯吡咯烷酮PVP、聚乙烯醇PVA和聚乙二醇PEG)改性后的聚偏氟乙烯(PVDF)超滤膜表面对牛血清蛋白(BSA)的动态吸附行为，用旋转涂覆法将膜固定在晶片表面，采用耗散型石英晶体微天平(QCM-D)研究不同改性膜表面上的蛋白质吸附行为以及吸附层的黏弹性随时间的变化规律.结果表明：BSA的吸附行为不仅与膜表面的亲水性有关，还受其表面化学性质的影响.不同亲水性条件下PVDF/PEG膜表面吸附量较少且不易清洗；对于PVDF/PVP和PVDF/PVA膜，随着亲水角减小，|-ΔD/Δf|值变化显著，吸附层的构象也发生明显的变化.不同添加剂所含的特殊官能团(—OH、—O—和—CON—)不仅能够降低膜表面污染物的吸附量，还能够影响吸附层的结构，易于清洗.
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| 关键词: 石英晶体微天平 聚偏氟乙烯超滤膜 牛血清蛋白 亲水性添加剂 官能团 |
| DOI：10.11918/j.issn.0367-6234.2016.08.013 |
| 分类号:X703.1 |
| 文献标识码:A |
| 基金项目:国家自然科学基金(8,8);陕西省科技统筹创新工程计划项目(2012KTCL03-6,3KTCL03-16) |
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| Adsorption behaviors of BSA on different modified PVDF ultrafiltration membranes using QCM-D |
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WANG Lei, HUANG Song, HUANG Danxi, WANG Xudong,Lü Yongtao, JIA Shifa
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(School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China)
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| Abstract: |
| To further unravel dynamic adsorption behavior of bovine serum albumin on the PVDF ultrafiltration membranes modified with three different additives (PVP, PVA or PEG), the membranes were fixed upon clean crystal by spin-coating, and the adsorption and cleaning of protein on different membrane surface were measured using quartz crystal microbalance with dissipation monitoring. It was found that the adsorption amount of BSA on PVDF/PEG membrane was relatively low, and the adsorbed layer structure was dense and difficult to remove by DI water cleaning. While for PVDF/PVP and PVDF/PVA membrane surfaces (especially PVDF/PVA), the conformation of the layers varied on later stage obviously, |-ΔD/Δf| values increased seriously, and the adsorption layers become looser. The results show that the absorbed mass of BSA is related to the hydrophilicity of membrane surfaces using the same additives, and the membranes modified with different additives show the different adsorption behaviors. Specific additives (—OH, —O—and—CON—) play a major role in determining the viscoelastic of absorption layer.
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| Key words: quartz crystal microbalance (QCM-D) PVDF ultrafiltration membrane BSA hydrophilic additive functional groups |
