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主管单位 中华人民共和国
工业和信息化部
主办单位 哈尔滨工业大学 主编 冷劲松 国际刊号ISSN 0367-6234 国内刊号CN 23-1235/T

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引用本文:刘文龙,刘超,沈琛,李夕耀,彭永臻.活性污泥长期好氧饥饿下的微生物种群结构演化[J].哈尔滨工业大学学报,2019,51(8):20-27.DOI:10.11918/j.issn.0367-6234.201808157
LIU Wenlong,LIU Chao,SHEN Chen,LI Xiyao,PENG Yongzhen.Microbial community evolution in activated sludge during long-term aerobic starvation period[J].Journal of Harbin Institute of Technology,2019,51(8):20-27.DOI:10.11918/j.issn.0367-6234.201808157
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活性污泥长期好氧饥饿下的微生物种群结构演化
刘文龙1,刘超2,沈琛2,李夕耀2,彭永臻1,2
(1.城市水资源与水环境国家重点实验室(哈尔滨工业大学),哈尔滨 150090; 2.北京工业大学国家工程实验室,北京市水质科学与水环境恢复工程重点实验室,北京 100124)
摘要:
为考察好氧饥饿环境对活性污泥硝化及除磷性能的影响,研究活性污泥在长期好氧饥饿条件下的微生物种群结构变化,以具有良好硝化和除磷性能的活性污泥为实验对象,利用Illumina高通量测序平台分别考察活性污泥好氧饥饿处理3,7,14和30 d后的微生物种群结构特性及差异. 结果表明:好氧饥饿时间越长,活性污泥硝化及除磷性能所受的影响越大,污泥的种群结构变化越明显. 硝化菌和除磷菌等相关功能细菌在短期(7 d)好氧饥饿过程中,可分别利用细菌衰亡裂解释放的氨氮和胞内储能物质进行细胞维持,确保系统硝化及除磷性能的恢复,同时恢复期氨氧化菌快于亚硝酸氧化菌的活性恢复速率促进了系统由全程硝化向短程硝化的转变;而随着好氧饥饿时间的延长,功能细菌的种群丰度均逐渐减少. 此外,活性污泥微生物种群结构在30 d好氧饥饿过程中经历了一个动态变化过程,既有优势种群(如Proteobacteria和Bacteroidetes等)的逐步消亡,又有适应好氧饥饿环境的菌种(如Firmicutes)增强成为新的优势菌群.
关键词:  活性污泥  好氧饥饿  衰减  恢复  高通量测序  微生物群落
DOI:10.11918/j.issn.0367-6234.201808157
分类号:X703
文献标识码:A
基金项目:水体污染控制与治理科技重大专项(2017ZX07103-003)
Microbial community evolution in activated sludge during long-term aerobic starvation period
LIU Wenlong1,LIU Chao2,SHEN Chen2,LI Xiyao2,PENG Yongzhen1,2
(1.State Key Laboratory of Urban Water Resource and Environment (Harbin Institute of Technology), Harbin 150090, China; 2.National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering (Beijing University of Technology), Beijing 100124, China)
Abstract:
Aiming at the effect of aerobic starvation environment on the nitrification and phosphorus removal performance of activated sludge and the variations of the microbial community evolution during long-term aerobic starvation periods, an activated sludge with high level removal (>99%) of ammonium and phosphorus was used to study the differences of microbial community structures by Illumina high-throughput sequencing technology during 3,7, 14, and 30 d aerobic starvation periods. Results showed that the performance of nitrification and phosphorus removal decreased with the increase of the aerobic starvation time, and the change of microbial community structure was more obvious with the proceeding of aerobic starvation. During short-term (7 d) aerobic starvation period, the functional bacteria including nitrifiers and phosphorus removing bacteria utilized the released ammonium from cell decay and intracellular storage polymers for cell maintenance to guarantee the recovery of nitrification and phosphorus removal performance. Besides, higher activity resuscitation rate of ammonium oxidizing bacteria (AOB) than nitrite oxidizing bacteria (NOB) contributed to the shift of the nitrification pathway from nitratation to nitritation. Furthermore, the abundances of functional bacteria gradually decreased with the prolonging of aerobic starvation time. The microbial community structures of the activated sludge underwent a dynamic change during the 30 d aerobic starvation period, in which the majority of the original dominant species within the phylum Proteobacteria and Bacteroidetes declined with a remarkable increase of Firmicutes that could acclimatize themselves to the aerobic starvation conditions.
Key words:  activated sludge  aerobic starvation  decay  resuscitation  high-throughput sequencing  microbial community

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