| 引用本文: | 刘涛,李冬,畅晓燕,曾辉平,曾涛涛,张杰.常温CANON反应器内微生物群落结构及生物多样性[J].哈尔滨工业大学学报,2013,45(8):28.DOI:10.11918/j.issn.0367-6234.2013.08.005 |
| LIU Tao,LI Dong,CHANG Xiaoyan,ZENG Huiping,ZENG Taotao,ZHANG Jie.Bacterial community structure and biodiversity of CANON reactor at room temperature[J].Journal of Harbin Institute of Technology,2013,45(8):28.DOI:10.11918/j.issn.0367-6234.2013.08.005 |
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| 摘要: |
| 为研究基于亚硝化的全程自养脱氮(CANON)工艺中微生物群落结构及种属特征,从常温条件下稳定运行的CANON反应器中采集生物膜样品,并基于聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术,分别对总细菌、氨氧化细菌和厌氧氨氧化菌进行群落结构解析．结果表明:反应器中与亚硝化作用有关的功能菌为亚硝化单胞菌属(Nitrosomonas)和亚硝化螺菌属(Nitrosospira),与厌氧氨氧化作用有关的功能菌是Candidatus Brocadia属和Candidatus Kuenenia属,未检测到亚硝酸盐氧化细菌,且系统内氨氧化细菌的生物多样性明显高于厌氧氨氧化菌．此外,反应器中还存在其他微生物,如Shewanella、Pseudomonas、Ignatzschineria、Dechloromonas以及属于Clostridia、α-Proteobacteria、Bacillales的uncultured bacteium等． |
| 关键词: 基于亚硝化的全程自养脱氮 16S rDNA 聚合酶链式反应-变性梯度凝胶电泳 微生物群落结构 生物多样性 |
| DOI：10.11918/j.issn.0367-6234.2013.08.005 |
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| 基金项目:国家自然科学基金资助项目(50878003);国家水体污染控制与治理科技重大专项(2008ZX07208-003-3,8ZX07208-005-3,9ZX07424-1,9ZX07424- 002-3,9ZX07424-005);北京市自然科学基金资助项目(8092006);城市水资源与水环境国家重点实验室(哈尔滨工业大学)开放基金项目(QAK201005). |
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| Bacterial community structure and biodiversity of CANON reactor at room temperature |
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LIU Tao1, LI Dong2, CHANG Xiaoyan2, ZENG Huiping2, ZENG Taotao1, ZHANG Jie1,2
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(1.State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 150090 Harbin, China; 2.Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, 100124 Beijing, China)
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| Abstract: |
| To investigate bacterial community structure and biodiversity of a CANON reactor that was operated stably at room temperature, total DNA was extracted from the biofilm for PCR-DGGE analysis. The results of microbial community analysis showed that the ammonia oxidizing bacteria Nitrosomonas and Nitrosospira, together with the anaerobic ammonia oxidizing bacteria Candidatus Brocadia and Candidatus Kuenenia were detected as the functional bacteria while nitrite-oxidizing bacteria (NOB) was not found in the CANON system, and the biodiversity of AOB was significantly higher than anammox bacteria. Besides, some other bacteria like Shewanella, Pseudomonas, Ignatzschineria, Dechloromonas and some uncultured bacterium related to Clostridia, α-Proteobacteria and Bacillales also existed in the reactor. |
| Key words: CANON 16S rDNA PCR-DGGE biocommunity structure biodiversity |
