| 摘要: |
| 为提高剩余污泥水解酸化过程中挥发性脂肪酸（VFAs）的累积,从剩余污泥中分离产蛋白酶活力较高的耐碱细菌,并构建产蛋白酶混合菌系. 将其接种于碱性（pH 10.0）发酵剩余污泥的不同发酵时期,评价其对溶解性有机化合物和VFAs累积的影响,探讨利用剩余污泥生产VFAs的最佳条件. 从剩余污泥中分离到2株产蛋白酶活力较高的耐碱细菌,并构建产蛋白酶混合菌系. 在发酵初期接种混合菌系效果最显著,且可缩短发酵启动时间2 d. 发酵初期接种混合菌系后,溶解性蛋白质和VFAs质量浓度在第8天均达到最高值,分别为未接种混合菌系样品中相应值的1.25和1.41倍,分别占溶解性化学需氧量(SCOD)总量的29.87%和44.54%. 乙酸和丙酸为剩余污泥水解酸化过程中VFAs的主要组分,分别占VFAs总量的50.69%和18.19%. |
| 关键词: 剩余污泥 水解酸化 混合菌系 内碳源 挥发性脂肪酸 |
| DOI：10.11918/j.issn.0367-6234.2014.12.006 |
| 分类号:X703 |
| 基金项目:国家高技术研究发展计划项目(2012AA6,1AA060903-02). |
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| Effect of mixed microbial consortium capable of protease-producing on hydrolysis and acidification of excess sludge under alkaline condition |
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JIE Weiguang1,2, PENG Yongzhen1,3
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(1.State Key Laboratory of Urban Water Resource and Environment(Harbin Institute of Technology), 150090 Harbin, China; 2. Dept. of Food and Environment Engineering, East University, 150086 Harbin, China; 3. Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering（Beijing University of Technology）, 100124 Beijing, China)
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| Abstract: |
| To improve volatile fatty acids (VFAs) accumulation from hydrolysis and acidification of excess sludge (ES), alkali-tolerant bacteria capable of protease-producing were isolated from ES. A mixed microbial consortium capable of protease-producing was constructed by the isolated bacterial strains. The mixed microbial consortium was inoculated into the different fermentation periods of ES to investigate their effects on soluble organic compounds and VFAs accumulation from ES under alkaline conditions (pH 10.0). The optimal condition for VFAs accumulation from ES was investigated. The results showed that two alkali-tolerant bacterial strains capable of protease-producing were isolated from ES and constructed as a mixed microbial consortium. The soluble organic compounds concentrations and VFAs accumulation were improved significantly after the mixed microbial consortium was inoculated at the initial fermentation, and the start-up phase was shortened by 2 days. On the 8th day of fermentation, the concentrations of soluble protein and total VFAs reached their peak values, and were 1.25 times and 1.41 times higher as compared to the corresponding values from non-inoculated samples, and accounted for 29.87% and 44.54% of total SCOD concentration, respectively. Acetic and propionic acids were the most prevalent VFAs (account for 50.69% and 18.19%, respectively). |
| Key words: excess sludge (ES) hydrolysis and acidification mixed microbial consortium internal carbon source volatile fatty acids (VFAs) |
