| 引用本文: | 张贺,刘晓为,揣荣岩,李新.用于Pb2+、Hg2+离子选择性固相萃取的微流控芯片[J].哈尔滨工业大学学报,2017,49(5):141.DOI:10.11918/j.issn.0367-6234.201701030 |
| ZHANG He,LIU Xiaowei,CHUAI Rongyan,LI Xin.Microfluidic chip for selective solid phase extraction of Pb2+ and Hg2+[J].Journal of Harbin Institute of Technology,2017,49(5):141.DOI:10.11918/j.issn.0367-6234.201701030 |
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| 摘要: |
| 为实现微流控芯片对重金属Pb2+、Hg2+离子的选择性固相萃取，首先采用3-氯丙基三甲氧基硅烷和5-甲基苯并三氮唑对纳米二氧化硅进行硅烷偶联改性，随后以改性纳米二氧化硅为基础，设计并制备了可更换吸附剂的一体化填充式固相萃取芯片.测试结果表明：改性后不仅吸附剂的团聚现象得到明显改善，且当pH=5时，对Pb2+的吸附率可达99.1%，对Hg2+的吸附率可达98.9%，而对Cr3+的吸附率为20.4%，对Mn2+的吸附率仅为13.2%.即使样品中混有干扰离子 (K+、Na+或Mn2+) 时，对Pb2+、Hg2+离子的吸附率仍均可达97%以上.当进样流速≤1.5 mL/min时，微流控芯片对Pb2+或Hg2+的吸附率可达98%以上；以0.5 mol/L的盐酸和2%的硫代尿素为洗脱液，控制流速在0.2~0.3 mL/min范围内时，芯片对Pb2+或Hg2+的洗脱率均达到92%以上.用于Pb2+、Hg2+离子的选择性固相萃取芯片的成功制备，有助于微流控技术在重金属污染监测领域的应用和推广.
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| 关键词: 微流控芯片 固相萃取 选择性“富集-分离” 纳米吸附剂改性 重金属离子 |
| DOI：10.11918/j.issn.0367-6234.201701030 |
| 分类号:TP273.4 |
| 文献标识码:A |
| 基金项目:国家自然科学基金(61372019) |
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| Microfluidic chip for selective solid phase extraction of Pb2+ and Hg2+ |
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ZHANG He1,LIU Xiaowei2,CHUAI Rongyan1,LI Xin1
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(1.School of Information Engineering and Science, Shenyang University of Technology, Shenyang 110870, China; 2.Key Laboratory of Micro-systems and Micro-structures Manufacturing (Harbin Institute of Technology), Ministry of Education, Harbin 150001, China)
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| Abstract: |
| To achieve the selective solid-phase extraction of Pb2+ and Hg2+ ions by microfluidic chip, the surface modification of nano-silica was carried out by using 3-chloropropyltrimethoxysilane and 5-methylbenzotriazole. And then, an integrated solid-phase extraction chip with replaceable adsorbent was designed and prepared based on the modified nano-silica. The results show that, the agglomeration of the adsorbent is improved significantly. When pH=5, the adsorption rates of Pb2+ and Hg2+ are 99.1% and 98.9% respectively, while the adsorption rates of Cr3+ and Mn2+ are only 20.4% and 13.2% respectively. In addition, the absorption rates of Pb2+ and Hg2+ are both more than 97% even when mixing with the interference ions (K+, Na+ or Mn2+). When the sample flow rate is no more than 1.5 mL/min, the microfluidic chip adsorption rate of Pb2+ or Hg2+ are both more than 98%; when using the hydrochloric acid (0.5 mol / L) and 2% thiourea [CS (NH2)2(W/W)] as eluent, the elution rate of Pb2+ or Hg2+ is both more than 92% (the flow rate is between 0.2~0.3 mL/min). The successful preparation of selective solid-phase extraction chips for Pb2+ and Hg2+ contributes to the application and popularization of microfluidic technology in heavy metal pollution monitoring.
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| Key words: microfluidic chip solid phase extraction selective separation and enrichment modification of nano-adsorbents heavy metal ion |
