| 引用本文: | 姚一欣,杨欣欣,敖誉旗,陈功,柳景青.户内供水管道特殊水力工况对微生物生长的影响[J].哈尔滨工业大学学报,2024,56(2):123.DOI:10.11918/202212005 |
| YAO Yixin,YANG Xinxin,AO Yuqi,CHEN Gong,LIU Jingqing.Effects of special hydraulic regimes of premise plumbing on the growth of microorganisms[J].Journal of Harbin Institute of Technology,2024,56(2):123.DOI:10.11918/202212005 |
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| 摘要: |
| 户内供水管道存在长时间滞留和间歇性用水的特殊工况,为此,以HZ市某小区内1 100户的时用水量数据为背景条件,搭建户内非循环管道模拟平台,设置高变化流量（HIG）、中等变化流量（MED）、低变化流量（LOW）、恒定流量（SS）和滞留168 h（RET）5种水力工况,探究户内供水管道特殊水力工况对管道微生物群落结构和条件致病菌的影响。运行3个月后收集33组生物膜样品和水样,开展实时荧光定量（real time qPCR）和16S rRNA基因测序分析,对样本中铜绿假单胞菌的绝对丰度进行定量并分析微生物群落结构组成和多样性。结果表明:RET水样细菌总数最大,且生物膜中活菌比例最高;间歇式用水工况下水样中含条件致病菌的菌属数量和丰度更高;HIG生物膜中细菌总数最大且含条件致病菌的菌属相对丰度最高为78.38%,HIG生物膜中铜绿假单胞菌拷贝数为4 798.81/cm2,是RET生物膜的7.59倍。RDA结果显示,浊度和pH分别是假单胞菌属和铜绿假单胞菌的主要环境影响因素。 |
| 关键词: 户内供水管道 水力工况 微生物群落结构 条件致病菌 |
| DOI：10.11918/202212005 |
| 分类号:TU991 |
| 文献标识码:A |
| 基金项目:国家自然科学基金(52070166) |
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| Effects of special hydraulic regimes of premise plumbing on the growth of microorganisms |
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YAO Yixin1,YANG Xinxin1,AO Yuqi1,CHEN Gong2,LIU Jingqing1
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(1.College of Civil Engineering and Architecture, Zhejiang University, Hangzhou 310058, China; 2.Power China Huadong Engineering Corporation, Hangzhou 311122, China)
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| Abstract: |
| Premise plumbing have special working conditions such as long-term detention of hydraulic regimes and intermittent water use. In this study, based on the hourly water consumption data of 1 100 households in a residential area of HZ City, an indoor non-circulating pipeline simulation platform was built, and five hydraulic regimes were set up: high varied flow (HIG), medium varied flow (MED), low varied flow (LOW), steady state (SS) and 168 h retention (RET), to explore the influence of special hydraulic conditions of premise plumbing on the microbial communities and opportunistic pathogens. After three months of operation, 33 sets of biofilm and water samples were collected, and real time qPCR and 16S rRNA gene sequencing analysis were performed. In addition, the absolute abundance of Pseudomonas aeruginosain the samples was quantified. The microbial community and diversity were analyzed. The results showed that the total number of RET water-like bacteria was the largest, and the proportion of viable bacteria in the biofilm was the highest. Conditionally pathogenic bacteria in the water samples has higher number and abundance under intermittent hydro-working conditions. The total number of bacteria in HIG biofilms and the highest relative abundance of conditionally pathogenic bacteria was 78.38%. The quantitative results of Pseudomonas aeruginosawere 4 798.81/cm2, which was 7.59 times that of RET biofilms. The RDA results showed that turbidity and pH were the main environmental factors for Pseudomonas and Pseudomonas aeruginosa respectively. |
| Key words: premise plumbing hydraulic regimes microbial communities opportunistic pathogens |
