| 引用本文: | 李冬,何永平,张肖静,梁瑜海,张玉龙,范丹,张杰.有机碳源对SNAD工艺脱氮性能及微生物种群结构的影响[J].哈尔滨工业大学学报,2016,48(2):68.DOI:10.11918/j.issn.0367-6234.2016.02.012 |
| LI Dong,HE Yongping,ZHANG Xiaojing,LIANG Yuhai,ZHANG Yulong,FAN Dan,ZHANG Jie.Effect of organic carbon on nitrogen removal and the microbial communities in SNAD process[J].Journal of Harbin Institute of Technology,2016,48(2):68.DOI:10.11918/j.issn.0367-6234.2016.02.012 |
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| 有机碳源对SNAD工艺脱氮性能及微生物种群结构的影响 |
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李冬1,何永平1,张肖静2,梁瑜海1,张玉龙1,范丹1,张杰1,3
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(1.水质科学与水环境恢复工程北京市重点实验室(北京工业大学),100124 北京; 2.郑州轻工业学院 环境污染治理与生态修复河南省协同创新中心,450001 郑州; 3.城市水资源与水环境国家重点实验室(哈尔滨工业大学),150090 哈尔滨)
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| 摘要: |
| 为考察不同有机碳源质量浓度对亚硝化的全程自养脱氮工艺（SNAD）脱氮性能的影响，将该工艺应用到生活污水的处理中，采用MBR反应器，以葡萄糖作为有机物来源，通过逐步增大COD来实现，并运用PCR-DGGE技术研究了微生物种群结构的变化.反应器运行结果和DGGE图谱分析表明：碳氮比为0~2时，COD的增加不会抑制AOB和Anammox菌，AOB和Anammox菌的菌属种类不受影响，反而通过反硝化作用提高氮去除负荷.总氮去除率和氮去除负荷分别为67%和0.34 kg/（m3·d）左右.碳氮比为3~4及生活污水运行条件下，Anammox菌不受影响，AOB的活性受到抑制，菌属种类减少，脱氮效率下降.生活污水运行阶段，总氮去除率和氮去除负荷平均分别为73%和0.17 kg/（m3·d）.Nitrosomonas和Candidatus Kuenenia stuttgartiensis一直是反应器内的优势菌属，共同完成脱氮过程.
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| 关键词: 同步亚硝化厌氧氨氧化和反硝化 有机物 生活污水 聚合酶链式反应-变性梯度凝胶电泳 微生物 |
| DOI：10.11918/j.issn.0367-6234.2016.02.012 |
| 分类号:X172 |
| 文献标识码:A |
| 基金项目:国家自然科学基金(51222807); 国家科技重大专项-水专项(2012ZX07202-005). |
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| Effect of organic carbon on nitrogen removal and the microbial communities in SNAD process |
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LI Dong1, HE Yongping1, ZHANG Xiaojing2, LIANG Yuhai1, ZHANG Yulong1, FAN Dan1, ZHANG Jie1,3
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(1.Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering(Beijing University of Technology), 100124 Beijing,China; 2.Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Zhengzhou University of Light Industry, 450001 Zhengzhou,China; 3.State Key Laboratory of Urban Water Resource and Environment(Harbin Institute of Technology), 150090 Harbin,China)
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| Abstract: |
| Effect of different organic substrate concentration on nitrogen removal in SNAD and the application of this process to treat domestic wastewater were investigated in a MBR, with gradually increased glucose as a source of organic matter. Besides, changes of microbial communities were observed by PCR-DGGE techniques. Experimental results and DGGE profiles showed that the increase of organic carbon concentration did not inhibited the activity and category species of AOB and Anammox bacteria when the C/N ration was ranged 0 to 2.0. Meanwhile, a significantly improvement of nitrogen removal was observed via effective denitrification, with a total nitrogen removal efficiency of 67% and nitrogen removal rate of 0.34 kg/(m3·d), respectively. Under the C/N of 3-4 condition, the anammox bacteria in the system changed insignificantly, but AOB was inhibited and the category species reduced, resulting a decreasing of nitrogen removal efficiency.Total nitrogen removal efficiency and nitrogen removal rate were about 73% and 0.17 kg/(m3·d), respectively.Nitrosomonas and Candidatus Kuenenia stuttgartiensis were the predominant microorganisms in the SNAD reactor and played a major role in autotrophic nitrogen removal.
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| Key words: SNAD organic carbon domestic wastewater PCR-DGGE microbes |
