| 引用本文: | 曾涛涛,李冬,曾辉平,张昭,李相昆,张杰.常温低基质亚硝化反应器功能菌群解析[J].哈尔滨工业大学学报,2016,48(2):76.DOI:10.11918/j.issn.0367-6234.2016.02.013 |
| ZENG Taotao,LI Dong,ZENG Huiping,ZHANG Zhao,LI Xiangkun,ZHANG Jie.Analysis of the functional bacteria community in partial nitrification reactor for low strength sewage treatment at ambient temperature[J].Journal of Harbin Institute of Technology,2016,48(2):76.DOI:10.11918/j.issn.0367-6234.2016.02.013 |
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| 常温低基质亚硝化反应器功能菌群解析 |
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曾涛涛1,李冬2,曾辉平2,张昭3,李相昆4,张杰2,4
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(1.污染控制与资源化技术湖南省高校重点实验室(南华大学),421001 湖南 衡阳; 2.水质科学与水环境恢复 工程北京市重点实验室(北京工业大学),100124北京; 3.中国建筑科学研究院建筑设计院,100013 北京; 4.城市水资源与水环境国家重点实验室(哈尔滨工业大学),150090哈尔滨)
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| 摘要: |
| 为维持亚硝化反应器稳定运行提供微生物理论基础，以常温（18~21.5℃）低基质推流式亚硝化反应器为对象，解析其稳定运行期间功能菌群特征.通过检测反应器三氮变化检验其亚硝化效果.利用扫描电镜（SEM）观察污泥微观结构，通过荧光原位杂交（FISH）、变性梯度凝胶电泳技术（DGGE）及克隆测序等方法，解析微生物菌群特性.保持反应器低溶解氧环境（0.1~0.6 mg/L），使氨氧化菌（AOB）竞争力强于亚硝酸盐氧化菌（NOB），在连续流运行80 d内，平均亚硝化率几乎为100%，出水NO2--N与NH4+-N质量比稳定在1.11.SEM结果显示，亚硝化污泥中球形细菌为优势菌群.FISH结果显示，AOB与NOB的相对比例分别为37.3%与4.4%.PCR-DGGE结果表明，反应器内存在6类优势微生物菌群，其中Nitrosomonas sp.为功能微生物AOB.由多种微生物组成的功能菌群维持反应器亚硝化稳定运行.
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| 关键词: 亚硝化 功能菌群 常温 低基质 氨氧化菌 亚硝酸盐氧化菌 |
| DOI：10.11918/j.issn.0367-6234.2016.02.013 |
| 分类号:X172 |
| 文献标识码:A |
| 基金项目:国家自然科学基金(51408293);中国博士后基金面上项目(2014M562114);湖南省教育厅优秀青年项目(14B154). |
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| Analysis of the functional bacteria community in partial nitrification reactor for low strength sewage treatment at ambient temperature |
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ZENG Taotao1, LI Dong2, ZENG Huiping2, ZHANG Zhao3, LI Xiangkun4, ZHANG Jie2,4
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(1.Hunan Province Key Laboratory of Pollution Control and Resources Reuse Technology( University of South China), 421001 Hengyang, Hunan, China; 2.Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering (Beijing University of Technology), 100124 Beijing, China; 3.Architectural Design Institute, China Academy of Building Research, 100013 Beijing, China; 4.State Key Laboratory of Urban Water Resource and Environment (Harbin Institute of Technology), 150090 Harbin, China)
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| Abstract: |
| Partial nitrification (PN) process was successfully developed in a plug flow reactor fed with low strength sewage at ambient temperature (18-21.5℃). For better detecting the efficiency and mechanism of the partial nitrification process in the PN reactor, ammonia, nitrite, and nitrate were detected firstly, subsequentially the functional bacterial community in the reactor was also investigated at microbial level. The sludge microstructures were detected by scanning electron microscopy (SEM), and the microbial characteristics was studied via fluorescence in situ hybridization (FISH), denaturing gradient gel electrophoresis (DGGE), cloning and sequencing analyzing. The DO concentration was maintained between 0.1 to 0.6 mg/L, to enhance AOB competition superiority. After 80 days continuous operation, PN reactor achieved nitrite accumulation rate of 100% and effluent NO2--N to NH4+ -N ratio achieved 1.11. SEM results showed that spherical bacteria were the predominant bacteria in partial nitrifying sludge. FISH results showed the proportion of AOB and NOB were 37.3% and 4.4%, respectively. Bacterial DGGE and sequencing results indicated that six dominant bacteria coexisting in the PN reactor, among which Nitrosomonas sp. were the main AOB species.Those multiple functional bacteria contributed to the nitrogen removal in PN reactor.
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| Key words: partial nitrification functional bacteria ambient temperature low strength sewage ammonia oxidizing bacteria nitrite oxidizing bacteria |
